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KMID : 0624620130460040201
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BMB Reports
2013 Volume.46 No. 4 p.201 ~ p.206
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Sulforaphane controls TPA-induced MMP-9 expression through the NF-κB signaling pathway, but not AP-1, in MCF-7 breast cancer cells
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Lee Young-Rae
Noh Eun-Mi
Han Ji-Hey
Kim Jeong-Mi
Hwang Bo-Mi
Kim Byeong-Soo
Lee Sung-Ho
Jung Sung-Hoo
Youn Hyun-Jo
Chung Eun-Yong
Kim Jong-Suk
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Abstract
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Sulforaphane [1-isothiocyanato-4-(methylsulfinyl)-butane] is an isothiocyanate found in some cruciferous vegetables, especially broccoli. Sulforaphane has been shown to display anti-cancerproperties against various cancer cell lines. Matrix metalloproteinase-9 (MMP-9), which degrades the extracellular matrix (ECM), plays an important role in cancer cell invasion. In this study, we investigated the effect of sulforaphane on 12-O-tetradecanoyl phorbol-13-acetate (TPA)-inducedMMP-9 expression and cell invasion in MCF-7 cells. TPA-induced MMP-9 expression and cell invasion were decreased by sulforaphane treatment. TPA substantially increased NF-κB and AP-1DNA binding activity. Pre-treatment with sulforaphane inhibited TPA-stimulated NF-κB binding activity, but not AP-1 binding activity. In addition, we found that sulforaphane suppressed NF-κB activation, by inhibiting phosphorylation of IκB in TPA-treated MCF-7 cells. In this study, we demonstrated that the inhibition of TPA-induced MMP-9 expression and cell invasion bysulforaphane was mediated by the suppression of the NF-κB pathway in MCF-7 cells.
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KEYWORD
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Invasion, MCF-7, MMP-9, NF-κB, Sulforaphane
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